SUMMARY, EXPLANATION AND LIMITATIONS:
Factor VIII or von Willebrand factor is a multimeric glycoprotein which bindes to platelet glycoprotein Ib, IIb/III, collagen and herapin. It is synthetized in endothelial cells and stored in cell Weibel-Palade bodies. It loss causes hemophilia A. It presents a granular reaction pattern, and can also be detected in megakaryocytes cytoplasm in human bone marrow sections. Factor VIII is a mediator of the platelets adhesion to the damaged vessel walls, is also the carrier and stabilizer of the coagulation factor VIII. Due to not all endothelial cells synthetize or store this molecule, is not possible detecting by staining the antigen related with Factor VIII in a 30% of vessels originated tumors, independently of its nature, malignant or benignant. The staining method with this antigen has also been used for determining the angiogenesis, an indicator of tumor recurrence.
Immunogen: Purified factor VIII related antigen / von Willebrand factor.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from skin, placenta or tonsil.
This antibody is designed for the specific localization of human factor VIII using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
It is used for characterizing endothelial cells or its derived in the vascularization and inflammatory lesions; diagnosing primary or secondary neoplasms originated in endothelium.