p27Kip1

Ref: AP10291 – Clone: DCS-72 – Host: Mouse – Method: P – Format: Ready to use

SKU: AP10291 Category: Tag:

Description

SUMMARY, EXPLANATION AND LIMITATIONS:
Cell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2.

Clone: DCS-72
Isotype: IgG1
Immunogen: BALB/C mice were injected with mouse recombinant p27Kip 1 protein.
Staining pattern: Nuclear.
Positive control: Tissue sample from prostate carcinoma.

APPLICATIONS:
This antibody is designed for the specific localization of human p27Kip1 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.