Anti-Nestin Antibody [Rat-401 (4D4)]

Dilution: IHC-Fr: 1:40 - 1:400, IHC-P: 1:40 - 1:400, We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.For ICC we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts.For WB it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.