SUMMARY, EXPLANATION AND LIMITATIONS:
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140kD. It is expressed in both the cytoplasm and cell membrane of Blymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia.
Immunogen: Recombinant Protein encoding the external domain of human CD22.
Staining pattern: Cell membrane.
Positive control: Tissue sample from hairy cell leukemia or tonsil.
This antibody is designed for the specific localization of human CD22 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.