SUMMARY, EXPLANATION AND LIMITATIONS:
The human Leukocyte Common Antigen (LCA) is a family of at least five glycoproteins, which are expressed on the cell surface of the majority of humane leukocytes. This antibody cocktail consists of two monoclonal antibodies directed against different epitopes of LCA. Each of both antibodies detects 94 – 96 % of human lymphocytes and monocytes. PD7/26 also reacts with Kupffer cells. The antibody cocktail labels lymphoid cells in tonsil, spleen, thymus and bone marrow. Additionally, mast cells stain positive. Neutrophils stain weakly positive; plasma cells are usually negative. Non-haematopoietic tissues do not react with this antibody cocktail. Both, B- and T-cells in non-Hodgkin lymphomas and B- and T-cell leukaemias of neoplastic tissues are labelled by this antibody cocktail. Hairy cells react positive whereas cells of granulocytic origin or cells from the erythrocytes lineage are mostly negative.
Immunogen: For PD7/26: humane peripheral blood cells, which are cultivated in the presence of T cell growth factor For 2B11: from T-cell lymphoma/leukemia isolated neoplastic cells.
Staining pattern: Cell membrane.
Positive control: Tissue sample from tonsil or lymph node.
This antibody is designed for the specific localization of human CD45 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Leukocyte common antigen (LCA/CD45) is located in the membrane of substantially all of the cells of the lymphoid and myeloid series. Therefore, the antibody can be used for immunohistochemical identification of neoplasms of the hematopoietic system and differentiation of carcinomas and sarcomas.
Additionally and as exceptionally described B and T lymphoma large cell positive for some type of cytokeratins and negative for CD45 and neuroendocrine carcinomas isolated positivity for CD45, it is advisable to use this antibody as part of a specific panel including at least one universal differentiation antigen B, T and a pancitoqueratina another broad spectrum.