SUMMARY, EXPLANATION AND LIMITATIONS:
CD68 is a transmembrane glycoprotein of unknown function highly expressed by human monocytes and tissue macrophages. CD68 transcripts are absent or present at very low levels in many hematopoietic lines including KG1, CEM, and K562, but can be induced by exposure to phorbol myristic acid. The extracellular domain consists of two distinct regions separated by an extended proline hinge: a membrane-distal mucin-like domain containing short peptide repeats and consisting of 54% serine and threonine residues; and a membrane proximal domain that has significant sequence homology to a family of lysosomal/plasma membrane shuttling proteins known as the lamp 1 group.
Immunogen: BALB/C mice were immunized with Gaucher’s cells.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from tonsil.
This antibody is designed for the specific localization of human CD68 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.