SUMMARY, EXPLANATION AND LIMITATIONS:
Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets (Langerhans’s cells). Chromogranin A is released as hormone in blood and can be used as biochemical marker. The chromogranin A immunoreactivity occurs in the majority of neuroendocrine cells but with variable intensity, length and intracellular distribution. Proteolysis of chromogranin A is produced by a family of endopeptidase (major PC1 / 3 and PC2) in the trans-Golgi system in the secretory granules and causing different peptides with biological activity (vasostatin, cromostatin, cromacin, parastatin, pancreastatin) . It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Immunogen: Recombinant protein encoding human chromogranin A.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from pancreas.
This antibody is designed for the specific localization of human Chromogranin A using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.