SUMMARY, EXPLANATION AND LIMITATIONS:
Cytokeratin 7 is an intermediate filament protein (IFP) of 54 kDa that recognizes the simple epithelium found in most glandular and transitional epithelia; but not that which is found in stratified squamous epithelia. This monoclonal antibody [clone OV-TL 12/30] is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, and is expressed in epithelial cells of ovary, lung, and breast, but not of colon or gastrointestinal tract. It is often used in conjunction with cytokeratin 20 in distinguishing ovarian, pulmonary, and breast carcinomas (CK7+) from colon carcinomas (CK7-).
Immunogen: Ovarian carcinoma cell line OTN11.
Staining pattern: Cytoplasmatic.
Positive control: Tissue sample from salivary gland, ovarian or breast carcinoma.
This antibody is designed for the specific localization of human cytokeratin 7 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.