SUMMARY, EXPLANATION AND LIMITATIONS:
Cytokeratins (CK) are intermediate filaments that constitute the cytoskeletal structure of virtually all epithelial but also of some non-epithelial cells. According to R. Moll they are ivided into Type I (acidic cytokeratins, CK9 to 20) and Type II (basic cytokeratins, CK1 to 8) cytokeratins. Each Type I cytokeratin is co-expressed with a Type II cytokeratin inside a single cell. Hence, it follows that all epithelial cells contain at least two different cytokeratins. Only CK19 is expressed unpaired.
The antibody of clone AE1 detects the acidic (Type I) cytokeratins 10, 15, 16 and 19. The antibody derived from clone AE3 detects all basic (Type II) cytokeratins, i.e. CK1 to 8. That means the antibody cocktail AE1/AE3 detects all cells containing cytokeratins and is therefore called “pan-Cytokeratin“ antibody. Detection of cytokeratins with a broad spectrum (“pan“-) antibody allows for the staining of epithelial cells in normal and abnormal tissues. It is especially useful in characterisation of metastases with unknown origin. Lerwill (2004) has shown that cocktail AE1/AE3 detects carcinomic metastases in lymph nodes more specific than other pan-CK antibodies.
Isotype: IgG1 + IgG1
Immunogen: Epidermal human cells.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from skin or squamous cell carcinoma.
This antibody is designed for the specific localization of human cytokeratin Pan using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody is used to determine the epithelial origin of tumors, especially in the case of metastatic character with the exceptions described in paragraph of specificity, interference and limitations.