SUMMARY, EXPLANATION AND LIMITATIONS:
IgG antibody reacts with the IgG human immunoglobulin γ-chain. The antibody marks IgG contained in all plasma cells and their precursors, most of them follicular dendritic cells, centroblastic-centrocytic lymph cells and IgG secretory multiple myelomas. Generally membrane bond immunoglobulins, connective tissue or blood vessels bond extracellular immunoglobulins and immunocomplex can only be shown on frozen tissues. Plasma cells may not be much stained on frozen tissues because immunoglobulins distribute diffusely by their cytoplasm.
Immunogen: IgG isolated from a pool of normal human sera.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from tonsil or lymph node.
This antibody is designed for the specific localization of human IgG using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody allows to determinate centroblastic-centrocytic lymphomas, multiple myeloma and plasma cells on inflammatory alterations.