SUMMARY, EXPLANATION AND LIMITATIONS:
This antibody reacts with the phosphorylated form of the three mains polypeptide subunits of human neurofilaments: low (68kDa) and high (200kDa) molecular weight. It also reacts with neurofilaments of rabbit, mouse, rat and cat. It does not react with dog neurofilaments. No other species tested.
Immunogen: Purified neurofilaments from human brain.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from cerebellum.
This antibody is designed for the specific localization of human neurofilaments using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Neurofilaments are complexes of macromolecules formed by three polypeptides named NF-L (68Kda), NF-M (155kDa) and NF-H (200kDa). They are localized in neurons axons of central and peripheral nervous system. Since neurofilaments are structural proteins, biochemically very stable, antibodies against them are very useful markers for studying neuronal expression, morphology, connectivity and pathology. The presence or absence of neurofilaments in a variety of neuroendocrine or nervous origin tumors provide an useful information about the cell type that constitute the tumor, particularly in neuroblastomas, medulloblastomas and retinoblastomas. The neurofilaments normal pattern is altered in some pathologies like Alzheimer disease, Lewy body disease, Parkinson and amyotrophic lateral sclerosis (Lou Gehrig disease). The expression pattern disorders also appears in most of sporadic or hereditary toxic induced axonopathies.