SUMMARY, EXPLANATION AND LIMITATIONS:
p53 (also known as tumor protein 53 [TP53]) is a transcription factor that regulates the cell cycle and, hence, functions as a tumor suppressor. p53 has been described as ”the guardian of the genome”, referring to its role in conserving stability by preventing genome mutation. p53 has many anti-cancer mechanisms. It can activate DNA repair proteins when DNA has sustained damage; it can also hold the cell cycle at the G1/S regulation point on DNA damage recognition. It can initiate apoptosis, programmed cell death, if DNA damage proves to be irreparable. p53 is central to many of the cell’s anti-cancer mechanisms. It can induce growth arrest, apoptosis and cell senescence. Mutations involving p53 have been found in a wide variety of malignant tumors, including Breast, Ovarian, Bladder, Colon, Lung, and Melanoma.
Immunogen: Recombinant human wild type p53 protein expressed in E. coli.
Staining pattern: Nuclear.
Positive control: Tissue sample from breast carcinoma or colon carcinoma.
This antibody is designed for the specific localization of human p53 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The expression of p53 is associated with poor prognosis in breast cancer. In colon tumors, p53 protein is expressed in 47% of cancers and 9% of adenomas only. It is expression in normal mucosa have found.