SUMMARY, EXPLANATION AND LIMITATIONS:
P504S or AMACR (α-Methylacyl-CoA-Racemase) is an enzyme, which is involved in β-oxidation of branched-chain fatty acids. It is expressed in prostatic adenocarcinomas but not in benign glands. Therefore, detection of P504S is helpful in distinguishing malignant from benign prostate glands. P504S is also detectable in most cases of HGPIN (high-grade prostatic intraepithelial neoplasia). Additionally, approximately 15-20% of AAH (atypical adenomatous hyperplasia) is expressing the enzyme. Recent investigations (A. Nassar et al. 2005) have shown that P504S is detectable in different levels in certain other tumours like colon, lung, endometrial and breast carcinomas as well as melanomas. Urothelial, kidney and liver cell carcinomas are also sometimes positive. p63 is detectable in benign basal cells of the prostate and in other epithelial tissues. Malignant tumours of prostate are negative for p63. Combined detection of P504S and p63 is very useful for the diagnosis of small prostate cancers in needle biopsies or HGPIN. Anti-P504S antibody stains positive and granular in cytoplasm of malignant and pre-malignant prostate glands whereas anti-p63 antibody stains positive in nuclei of benign glands.
Clone: Polyclonal + Y4A3
Isotype: Rabbit IgG (p504S) and mouse IgG2a (p63)
Staining pattern: Granular cytoplasmic (p504S) and nuclear (p63) in epithelial cells.
Positive control: Tissue sample from prostate carcinoma.
This antibody is designed for the specific localization of human proteins p504S and p63 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The combined detection of P504S and p63 is useful for the diagnosis of small prostate cancer in fine needle aspirations or HGPIN. Anti-P504S stain positively and in the cytoplasm of cells granules of premalignant and malignant prostate glands, while the anti p63 stains the nuclei of cells in benign glands.