SUMMARY, EXPLANATION AND LIMITATIONS:
Simian virus SV40 has provided an important model for studies of cellular mechanisms involved in a malignant transformation. The major SV40 translational products include the Large T antigen and the Small T antigen, both of which are encoded by the early region of the SV40 viral genome. The Large T antigen complexes with the p53 suppressor gene, resulting in its functional inactivation, thus promoting cell transformation. In addition, SV40 Large T antigen binds DNA polymerase and the transcription factor AP-2. It also forms complexes with a second tumor supressor gene-encoded protein, Rb 105. Binding of SV40 T antigen is specific for the “pocket” domain of Rb p105, which is also the binding site for the E2F cellular transcription factor.
Clone: Pab 101
Immunogen: Peptide mapping at the C-term of SV40 T Ag.
Staining pattern: Nuclear.
Positive control: Tissue sample infected with SV-40.
This antibody is designed for the specific localization of human SV-40 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.