SUMMARY, EXPLANATION AND LIMITATIONS:
Thyroid transcription factor-1 (TTF-1) is also known as thyroid-specific enhancer-binding protein (T/EBP). It is a 40 kDa protein which belongs to the NKx2 homo domain transcription factor family and regulates specific genes in thyroid glands and lungs. TTF-1 is a selective marker for adenocarcinomas of these tissues. It stains positive in nuclei of epithelial cells of thyroid glands and lung. Other organs are TTF-1 negative. TTF-1 antibody is extremely useful to differentiate between adenocarcinomas of lung and metastatic breast carcinomas and mesotheliomas. Nakamura et al. (2001) detected TTF-1 in fetal and adult hypothalamus of rats. Moreover, the antibody of clone 8G7G3/1 reacts with an unknown antigen in the cytoplasm of hepatocytes. Due to this side-reaction the antibody can be used for differential diagnosis of liver cell tumours. This was shown by several studies (amongst others: Lei et al. 2006).
Immunogen: Recombinant rat TTF-1 protein.
Staining pattern: Nuclear.
Positive control: Tissue sample from thyroids or lung.
This antibody is designed for the specific localization of human TTF-1 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.