SUMMARY, EXPLANATION AND LIMITATIONS:
Uroplakins Ia, Ib, II and III are structural proteins of terminal differentiated urothelial cells. In normal urothelia they are expressed in the luminal cytoplasmic membrane of umbrella cells. The antibody of clone AU1 reacts specifically with Uroplakin III. In formalin-fixed paraffin sections Uroplakin III can be detected in 50 – 60 % of primary urothelial carcinomas und metastatic carcinomas of urothelial origin. Uroplakin III is known as specific urothelial differentiation marker in the context of metastatic carcinomas of unknown origin. Benign
Brenner tumours, but not transitional cell carcinomas of the ovary, are the only exceptions from urothelial expression of Uroplakin III described so far. The immunohistochemical detection of Uroplakin III often results in irregular allotted signals, sometimes only with focal positivity. Additionally, cytoplasmic staining of varying intensity may occur besides the membrane staining.
Immunogen: Asymmetric unit membrane (AUM-) preparation from bovine bladder.
Staining pattern: Urothelial surface membrane.
Positive control: Tissue sample from urothelium.
This antibody is designed for the specific localization of human uroplakin III using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The detection of this antigen is used to detect primary urothelial carcinomas as well as for detection of metastatic carcinoma of urothelial origin.